Fluorescence of catechol amines and related compounds condensed with formaldehyde. Investigation of the detoxification mechanism of formaldehyde-treated tetanus toxin. Malkis, G.L. Heinz Fraenkel-Conrat, Jane Fraenkel-Conrat. Effects of modification of ε-amino groups on the interaction of κ- and. Arthur S. Brecher, Alan P. Koterba, Michael H. Basista. Huizhi Fan, Huimin Bao, Luyan Zhang, Gang Chen. Alan P. Koterba, Steve Smolen, Attaya Joseph, Michael H. Basista, Arthur S. Brecher. Advanced Concepts in Fixation: 1. E. Chiellini, F. Chiellini, P. Cinelli. Jonathan R. Catazaro, Arthur S. Brecher. Introduction to the Theory and Practice of Fixation of Tissues. La détoxication des toxines protéiques par le formol: Mécanismes supposés et nouveaux développements. Synthesis and mechanical properties of new fibre-reinforced composites of inorganic polymers with natural wool fibres. Rudy G. E. van Eijsden, Catherine Stassen, Luk Daenen, Sebastiaan E. Van Mulders, Prashant M. Bapat, Verena Siewers, Katty V. Y. Goossens, Jens Nielsen, Freddy R. Delvaux, Paul Van Hummelen, Bart Devreese, Ronnie G. Willaert. Han-Yuan Liu, Hannah Grant, Hung-Lun Hsu, Raya Sorkin, Filip Bošković, Gijs Wuite, and Susan Daniel . Springer Nature is developing a new tool to find and evaluate Protocols. Formaldehyde cross-linking of protein complexes combined with immunoprecipitation and mass spectrometry analysis is a promising technique for analysing protein-protein interactions, including those of transient nature. The same crosslinking methods that are used by manufacturers of conjugated secondary antibodies can be applied at a smaller scale by individual researchers for many kinds of protein-protein conjugation needs. However, glutaraldehyde is a very versatile reagent. Towards a Molecular Dimension in Histology. An example procedure for this is shown below (there is much information on this procedure on internet websites). Stable, nonreducible cross-links of mature collagen. Pilar Hernández-Muñoz, Ricardo Villalobos, Amparo Chiralt. The pH-Dependence of Ester Hydrolysis. Effect of formaldehyde on protein cross-linking and gel forming ability of surimi from lizardfish induced by microbial transglutaminase. [19] Sensitive detection of proteins and peptides in polyacrylamide gels after formaldehyde fixation. Xilong Wu, Chaoliang He, Yundi Wu, Xuesi Chen. II. Heat stability of milk: the mechanism of stabilization by formaldehyde. The adsorbed derivative on MANAE-agarose prepared as previously described was incubated with 0.5% glutaraldehyde solution (unless otherwise specified) in 25 mM sodium phosphate buffer at pH 7 and 25 °C for 1 h, under mild stirring. Kodela Vani, Steven A. Bogen, Seshi R. Sompuram. Leong, Trishe Y.-M. Leong. Titova, Ye.M. Jos J. Effect of gelatin-immobilization on the catalytic activity of enzymes and microbial cells. Enhanced pattern resolution, swelling-behaviour and biocompatibility of bioimprinted casein microdevices. Mateo C., Torres R., Fernández-Lorente G., et al. Belavtseva. Tissue Cell Sample Preparation: Lessons from the Antigen Retrieval Technique. The Nature and Limitations of Electron Microscopic Methods in Biology. New Dimensions of Antigen Retrieval Technique. Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues. Azadeh Hashemi, Fanny de Decker, Louise Orcheston-Findlay, M. Azam Ali, Maan M. Alkaisi, Volker Nock. Microelectrophoretic Studies on the Surface Chemistry of Erythrocytes. Cross-Linking Applications Protein Structural & Functional Studies Cross-linking agents are used to study the structure and composition of protein molecules. Antigen Retrieval Immunohistochemistry: Practice and Development. Thanh T. K. Vuong, Christine Vever-Bizet, Stéphanie Bonneau, Geneviève Bourg-Heckly. Whole aortic valves were exposed to 0.5% glutaraldehyde solution for 0, 1, 15, and 60 minutes, 6 hours, and 1 and 7 days.